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The Windows Registry is a hierarchical database that stores low-level settings for the Microsoft Windows operating system and for applications that opt to use the registry. The kernel , device drivers , services , Security Accounts Manager , and user interface can all use the registry. The registry also allows access to counters for profiling system performance. In simple terms, the registry or Windows Registry contains information, settings, options, and other values for programs and hardware installed on all versions of Microsoft Windows operating systems. For example, when a program is installed, a new subkey containing settings such as a program's location, its version, and how to start the program, are all added to the Windows Registry. When introduced with Windows 3.
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Riboswitches are highly structured elements residing in the 5' untranslated region of messenger RNAs that specifically bind cellular metabolites to alter gene expression. While there are many structures of ligand bound riboswitches that reveal details of bimolecular recognition, their unliganded structures remain poorly characterized. Characterizing the molecular details of the unliganded state is crucial for understanding the riboswitch's mechanism of action because it is this state that actively interrogates the cellular environment and helps direct the regulatory outcome. To develop a detailed description of the ligand free form of an S -adenosylmethionine binding riboswitch at the local and global levels, we have employed a series of biochemical, biophysical, and computational methods. Our data reveals that the ligand binding domain adopts an ensemble of states that minimizes the energy barrier between the free and bound states to establish an efficient decision making brachpoint in the regulatory process. Riboswitches are cis-acting mRNA regulatory elements that modulate gene expression through their ability to bind small molecule metabolites with high specificity Montange and Batey, ; Roth and Breaker, Ligand binding to a receptor, called the aptamer domain, redirects the folding outcome of the 5'-untranslated region of the mRNA, thereby determining the expression fate of the mRNA Garst and Batey,